The cell line and plasma sEV-associated proteins uncovered in this study are also known effectors of cancer-associated mechanistic regulators including transcription factors TP53 and MYC; signal transducer KRAS; cell growth and proliferation cytokines TGFB1, HGF, VEGF and EGF; and proinflammatory and/or immunoregulatory cytokines TNF, IL1B, IL6 and IFNG, all with established, fundamental roles in cancer etiology. The gene discussed is TNF; the disease is cancer.