While our study clarifies that IFITM3 does not contribute significantly to the IFN-I response in A549 cells, we cannot rule out that IFITM3 inhibits ZIKV at baseline levels and/or as a part of the IFN-I response in other cell types, as suggested by results of previous studies showing enhancement of ZIKV infection following shRNA-mediated knock down of IFITM3 (HeLa, HDFa) and IFITM3-knockout (MEF) [15,16]. This evidence concerns the gene IFITM3 and Zika virus infectious disease.