Twenty‐three genes with significant changes were enriched in GSEA, and seven genes showed consistent tendency across the three HCC cell line samples, including PIK3C3, RELA, FOXO3, MTOR, ATG5, MAP3K7, and MAPK1 (Fig 4A and Appendix Fig S4A). The gene discussed is ATG5; the disease is hepatocellular carcinoma.