Upon stimulation with a HER2-positive tumor cell line (OVCAR-3), DARPin-28z-, DARPin-BBz-, and DARPin-z-T cells all showed a similar capacity to produce interferon (IFN)-γ (Figure 1C, closed symbols) and tumor necrosis factor alpha (TNF-α) (Figure 1D, closed symbols); these CAR-T cells were not stimulated by the HER-2-negative line, LOX-IMVI (Figures 1C and 1D, open symbols). The gene discussed is TNF; the disease is neoplasm.