The expression of MTHFD1L in CRC cell lines (HCT116, LS174T, LOVO, DLD-1, SW620, SW480, HCT-8, HCT-8, HT-29) was shown in the figure 3A, and the NCM460 cells were used as the normal control. The gene discussed is MTHFD1L; the disease is colorectal carcinoma.