CD274 and neoplasm: In order to further study the status of PD-L1 expression on tumor cells, we used flow cytometry and immunofluorescence (IF) staining and found that neither anlotinib, bevacizumab or the combination of them had any significant effects on PD-L1 expression in non-VECs in tumor tissues, nor on PD-L1 expression in tumor cells (Fig. 5f and Fig. S2c).