Inoculating these intermediates onto human hepatoma (Huh-7) cells at a multiplicity of infection (MOI) of 0.1 produced gRNA levels (Fig. 1B) and infectious viral progenies (Fig. 1C) that were intermediate to those of the control PR1 and PR2B viruses at 24 h postinfection (hpi). The gene discussed is TMEM37; the disease is infection.