The significant ROH segment in BTA8 harbors at least 3 putative genes related to male fertility, namely PIP5K1B, FXN, and TJP2. Gene PIP5K1B along with PIP5K1A play an important role in spermatogenesis in mice, since knockout males lacking both proteins exhibit complete infertility due to decreased sperm number and a 25% reduction in testes weight compared to wild-type males [36]. The gene discussed is TJP2; the disease is Infertility.