Hepatitis B markers were diagnosed with a significantly higher frequency in Roma living in segregated settlements: HBsAg positivity was found in 12.4% of Roma and 2.8% of non-Roma (RR: 4.468 (2.373–8.415); p < 0.0001) and anti-HBc IgG positivity in 52.8% of Roma and 15.9% of the non-Roma population (RR: 3.13 (2.598–4.224); p < 0.0001). Here, PPIB is linked to hepatitis A virus infection.