CD274 and neoplasm: Importantly, for both methods (ULTIVUE, Figure 3A,B and OPAL, Figure 3C,D), PD-L1+/CD68+ macrophages could be readily identified within nests of strongly positive PD-L1+/CK+ (Figure 3 column A,C) or negative PD-L1-/CK+ tumor cells (Figure 3 column B,D).