Cell cycle analysis based on Ki-67 expression and DNA content (DAPI) (exemplified in Figure 1C) revealed an enrichment of quiescent cells among normal HSPCs from +AML groups (3.3-fold and 1.6-fold increase compared with CD34+-alone for +AML cell lines and +AML patient samples, respectively) (Figure 1D). This evidence concerns the gene CD34 and acute myeloid leukemia.