Moreover ROC curves identified the best cut-offs of RHI (AUC =0.772; p = 0.0001; sensitivity =65%, specificity = 85%, cut-off value ≤ 1.75), IL-18 (AUC=0.906; p= 0.0001; sensitivity = 81%, specificity = 97.52%, cut-off value> 269.9), TNF-α (AUC = 0.904, p = 0.0001; sensitivity = 80%, specificity = 99.17%, cut-off value> 5, 55), sIL-2Rα (AUC = 0.895; p = 0.0001; sensitivity = 74.75%, specificity = 100%, cut-off value> 511) for the presence of AF (Figure 2). The gene discussed is TNF; the disease is atrial fibrillation.