Although a thorough investigation of the expression of the different isoenzymes (LDHA and LDHB) and MCTs (MC1 ad MCT4) in the two, more aggressive prostate cancer cell lines (PC3 and DU145) would be needed, it can be concluded that the experiments reported herein have shown that the polarization obtained on [1-13C]pyruvate, as obtained using the PHIP-SAH methodology is more than sufficient for investigation of the differences in the metabolic phenotype of prostate cancer cells characterized by different aggressiveness (LNCaP, PC3, and DU145). Here, LDHA is linked to Familial prostate cancer.