In addition, comparing the five genes against the distribution of all genes, PGS1 and CRLS1 have significantly smaller CERES gene dependency scores (Student’s t-test, pPGS1 < 2.2 10–16 and pCRLS1 = 0.01455), which indicates that these two genes are particularly essential for glioma cell lines and together with our model based predictions, suggests they could be promising novel drug targets. Here, PGS1 is linked to central nervous system cancer.