We have previously observed that anti-EGFR-targeted therapy in vitro is capable of inducing immediate transcriptional changes in the HaCaT keratinocyte cell line model with constitutive EGFR activation.10 Here, we corroborate this observation by showing that two HNSCC cell lines also present immediate changes to cetuximab and in pathways relevant for resistance. The gene discussed is EGFR; the disease is head and neck squamous cell carcinoma.