Overall, the method proposed to generate muscle cells from patient-specific iPSC is particularly suitable to study aberrant activation of pathogenic pathways (i.e., TGFβ and other fibrotic signaling) in DMD muscles and can be exploited to investigate the molecular basis of DMD and the identification of molecular targets for interventions that counter the fibrogenic activity of DMD muscles. This evidence concerns the gene TGFB1 and Duchenne muscular dystrophy.