Given the increased number of mitochondria with Ca2+ overload in APP/PS1 Tg mice after amyloid plaque deposition, we performed Western blots for MCU, MiCU1, MiCU2, and NCLX in mitochondrial-enriched fractions of the brain of 10-mo-old Wt and APP/PS1 Tg mice to test whether Aβ affects the expression levels of these proteins. The gene discussed is APP; the disease is amyloidosis.