As shown in Fig. 5a, knockdown of BECN1 increased the accumulation of LC3B-II (an marker of the autophagosome) and SQSTM1/p62 (a marker of autophagic flux) in both LoVo and SW48 cells, indicating that BECN1 might play an important role in autophagic flux in CRC. This evidence concerns the gene SQSTM1 and colorectal carcinoma.