In order to further explore the molecular mechanism that LINC01116 promoted the malignant progression of glioma cells, we performed high-throughput sequencing of the transcriptome on Ln229 cells with or without knockdown of LINC01116 and found that 715 genes were differentially expressed (>2-fold change, P < 0.05, false discovery rate (FDR) < 0.05) (Fig. 3a and Supplementary Table S3). The gene discussed is LINC01116; the disease is central nervous system cancer.