TRAF4 and colorectal carcinoma: The interaction between CHK1 and TRAF4 was confirmed by co-immunoprecipitation (Co-IP) of ectopically expressed proteins in 293T cells (Fig. 1b), and endogenous binding was confirmed via reciprocal IP in both HT29 (Fig. 1c) and SW620 (Fig. S1a) CRC cells.