Impaired cell migration as measured by chemotaxis in transwell migration assays (Fig. 3g) as well as attenuated tumorsphere formation were also observed using the previously described GR-null/low U2OS osteosarcoma cell line engineered to stably express S134A-GR relative to wt-GR (Fig. 3h) [15]. This evidence concerns the gene NR3C1 and osteosarcoma.