Furthermore, we demonstrated that GLUT1 was an important intermediate component in ACSL4-induced O-GlcNAcylation, HCC cell proliferation, tumorigenesis and apoptosis inhibition, suggesting that ACSL4 promoted HCC growth via GLUT1-mediated O-GlcNAcylation elevation. This evidence concerns the gene ACSL4 and hepatocellular carcinoma.