Therefore, we applied the transwell migration assay with NSCLC cells seeded in the lower chamber and PMA‐treated THP‐1 or primary B6 macrophages (Mφ) seeded in the upper wells (Fig. 2A) to examine the impact of NSCLC ERα expression on the recruitment of macrophages. Here, ESR1 is linked to non-small cell lung carcinoma.