Then, inhibitor of ERK phosphorylation (U0126, 10 μm), inhibitor of Akt phosphorylation (MK‐2206, 1 μM) (Zhang et al., 2015), as well as the specific antagonist of CXCR4 (AMD3100, 1 μM) (Hatse et al., 2002), were added into THP‐1‐CM, and results show that they can all efficiently reverse CM‐increased ERα expression in the lung cancer cells (Fig. 7D). This evidence concerns the gene ESR1 and lung cancer.