RAB27A and neoplasm: To understand how expression of Rab27a at the primary tumor site affects metastatic outgrowth, we have designed experiments, where scr-KPC or shRab27a-KPC cells were orthotopically injected into pancreata of WT mice, and then GFP-scr-KPC or GFP-shRab27a-KPC cells were injected intrasplenically 2 weeks post-orthotopic injection, at the time when we know that the immune niche at the distant organ sites has been established (Fig. 5A,B).