Focusing on the α-MSH molecular mimicry, the families that most contributed to the ClpB-like gene function (Fig. 3a) and were also detected in relative lower abundance in subjects with obesity (Fig. 3b–d), such as not assigned Firmicutes, Rikenellaceae, and Clostridiaceae showed 48,192 and 175 sequences with 100% homology with α-MSH epitope of E. coli with a total mean of identity of 82.13%, 92.25%, and 81.36%, respectively (Additional files 2-3). The gene discussed is STAMBP; the disease is obesity due to melanocortin 4 receptor deficiency.