To determine the intracellular status of GLI-mediated signaling in hepatoma cell lines, a gene expression analysis of GLI1, GLI2, and GLI3 was performed on a panel of hepatoma cells including three differentiated (HepG2, HuH1, and HuH7) and two undifferentiated (HLE and HLF) types of HCC cell. This evidence concerns the gene GLI2 and hepatocellular carcinoma.