HT29 colon cancer cells are widely used to detect necroptosis by co-treatment with TNFα, BV6 (IAP inhibitor) and z-VAD-fmk (caspase inhibitor) [31], and MLKL (mixed lineage kinase domain like pseudokinase) phosphorylation is used as a marker of necroptosis [32]. Here, TNF is linked to colonic neoplasm.