Western blot analysis and RT-qPCR were used to detect the mRNA and protein expression of some representative tumor stem cell markers (Sox-2, Oct-4, Nanog, Aldh1, Slug,) and our results showed that the mRNA and protein expression of tumor stem cell markers in the sh-JADE-1 group was significantly higher than that in the sh-NC group. Here, NANOG is linked to neoplasm.