Supernatants of formed granulomas were collected on Day 3 and analyzed for IL-2R, IL-6, IL-7, IL-10, IL-12, IFN-γ, TNF-α, GM-CSF, CCL2, CCL3, CCL4, CCL5, CXCL9, CXCL10 and CCL11 cytokine concentrations using Illumina multiplex ELISA according to the manufacturer’s recommendations. This evidence concerns the gene TNF and Granuloma.