However, no significant difference was found for variant function between these disorders, except between PHTS and Somatic Cancer in the HEK293 pAKT/AKT assay (Supplementary Fig. 6a), but this difference was lost when variants we determined to be likely benign due to WT stability and function in multiple models (below) were removed from analysis (Supplementary Fig. 6b). Here, AKT1 is linked to PTEN hamartoma tumor syndrome.