In addition, we assessed dermatitis severity, histological features, infiltration of inflammatory cells such as mast cells and CD4+ T cells, expression of interleukin (IL)-4 and intercellular adhesion molecule-1 (ICAM-1), and stress-related hormone and neurotransmitter levels for evaluation of the anti-AD effect of NI-01 in the house dust mite extract-induced AD model using NC/Nga mice. The gene discussed is IL4; the disease is Alzheimer disease.