We tested DHMEQ, for the first time, on HCC cell lines (HA22T/VGH, HepG2, and HuH-6), in which the molecule produced cytotoxic and pro-apoptotic effects in a proportional manner to levels of constitutively activated NF-κB in the three cell lines. This evidence concerns the gene NFKB1 and hepatocellular carcinoma.