Furthermore, MUC1-specific CAR-T cells significantly increased secretion ofIL-2 (597.5 ± 38.89 pg/mL, 15-fold, Fig.5B), TNF alpha(359 ± 1.41 pg/mL, 6-fold, Fig.5E) and IFN-γ (182 ± 9.89pg/mL, 9-fold, Fig.5H) in response to MCF-7, anotherMUC1 positive tumour cell line. Here, TNF is linked to neoplasm.