When MUC1-negative tumour cells (A431)were used as the stimulator, MUC1-specific CAR-T cellsshowed no significant secretion of IL-2 (30 ± 2.82 pg/mL,1-fold, Fig.5C), TNF alpha (8 ± 2.82 pg/mL, 1.7-fold,Fig.5F) and IFN-γ (22 ± 2.82 pg/mL, 1.2-fold, Fig.5I). This evidence concerns the gene TNF and neoplasm.