To estimate the biologic impact of NETs on T cells, the canonical inducers of T1D, we co-cultured PBMCs with 1,000 ng/ml autologous NET fragments and then evaluated the delineation of T cell populations into Th1 cells (IFNγ-producing CD4 T cells), Th17 cells (IL-17A-producing CD4 T lymphocytes), T regulatory lymphocytes (Treg, FoxP3+CD25hiCD127– CD4 T cells) and IFNγ-producing CD8 lymphocytes. Here, CD8A is linked to type 1 diabetes mellitus.