The tumor growth rate in mice treated with EGF-PLGA@5Fu/PFC NPs was significantly lower than that in the groups treated with free 5Fu, PLGA@5Fu NPs and PLGA@5Fu/PFC NPs, and the different formulations that contained 5Fu induced a trend toward a decrease in tumor growth rate (Fig. 4b). The gene discussed is EGF; the disease is neoplasm.