As shown in Fig. 2, the fluorescence intensity of the non-pretreated cells incubated with EGF-PLGA@5Fu/PFC NPs was stronger than that of non-targeted NPs (PLGA@5Fu/PFC), and uptake increased in a time-dependent manner, which suggested greater adsorption of EGF-PLGA@5Fu/PFC NPs to colon cancer cells. Here, EGF is linked to malignant colon neoplasm.