We have observed that high expression levels of PAI1 are associated with increased metastasis and invasion in rectal tumors of the HUVR-IBIS and TCGA cohorts, respectively, and that, accordingly, it is highly significantly correlated with EMT-associated gene expression in the latter database and in the GSE35452 (Figure 2 and Figure 3). This evidence concerns the gene SERPINE1 and rectal neoplasm.