Moreover, high expression levels of PAI1 are associated with increased metastasis and invasion of rectal tumors in this cohort and TCGA database, respectively (Figure 3), which is correlated with EMT-associated and drug target genes expression, including PDGFRa, PDGFRb, FYN, PIM1 and BRAF (Figure 2 and Figure 4). This evidence concerns the gene SERPINE1 and rectal neoplasm.