Therefore, we generated a set consisting of granulocyte-macrophage colony-stimulating factor (GM-CSF) (M1) and M-CSF (M2) differentiated macrophages from six healthy blood bank donors which were either infected with Mtb-H37Rv, stimulated with LPS (100 ng/ml) or left untreated and subsequently harvested at either 4 or 24 h post-infection for metabolite extraction. This evidence concerns the gene CSF1 and infection.