ACTG1 and deafness: To understand the putative disease-causing mechanisms, the intracellular localization of wild-type and mutant γ-actins with deafness-associated variants (ACTG1:NM_001614: c.102 C > G: p.I34M, c.110 G > A: p.R37H, c.142 G > C: p.G48R, c.246 G > A: p.M82I, c.354 G > C: p.K118N, c.493 C > G: p.I165V, c.721 G > A: p.E241K and c.823 C > T: p.H275Y) was analyzed by expression assay utilizing the NIH/3T3 fibroblast cell line.