Biological pathways related to fatty acid metabolism (P= 0.0034), cell cycle (P= 0.00112), bile acid and bile salt metabolism (P= 0.00326), and PLK1 signaling pathway (P= 0.00554) were enriched in the HCC cells with NUDT1 overexpression (Figure 5A, Supplementary Table 4). This evidence concerns the gene NUDT1 and hepatocellular carcinoma.