Flies depleted of Akirin (C564-Gal4/UAS-RNAi-akirin), Relish (C564-Gal4/UAS-RNAi-relish) or Hyd (C564-Gal4/UAS-RNAi-hyd1 or C564-Gal4/UAS-RNAi-hyd2) displayed an impaired survival following E. coli infection when compared to control flies (C564-Gal4/UAS-RNAi-GFP) or to PBS pricking (Fig 2B and S2 Fig). Here, LGALS4 is linked to escherichia coli infection.