NKX2-5 and infection: Notably, at the highest simulated infection level of 600 μM cA4, Csx1 persists in a 100% activated form for over 1200 min in the absence of AcrIII-1, but is reduced to 50% activated within 150 min in its presence, with similar effects seen at lower cA4 concentrations (Figure 6—figure supplement 2).