Plasma methylation analysis of the four genes panel with APC, glutathione S-transferase P 1 (GSTP1), RASSF1A, and secreted frizzled-related protein 1 (SFRP1) resulted in an increased accuracy of 93% to differentiate between HCC and healthy controls (Huang et al. 2011). This evidence concerns the gene RASSF1 and hepatocellular carcinoma.