We also detected proliferation‐, migration‐ and EGFR/PI3K/AKT/mTOR‐related proteins by Western blotting, finding that alterations in cyclin E1, cyclin E2, CDK2, c‐Myc, RhoA, RhoC, P‐EGFR (Tyr1068), P‐AKT (Ser473) and P‐mTOR (Ser2448) protein levels were consistent with TPO expression in EGF‐stimulated NSCLC cells, whereas the opposite effect was observed on P27 levels, as expected (Figure 6D,E). This evidence concerns the gene AKT1 and non-small cell lung carcinoma.