Notwithstanding a striking downregulation of the checkpoint factors at the transcript (Supplementary Material, Fig. S4B–F) and protein levels (Fig. 4), CIN− (DLD1) or the CIN+ (SW480) colorectal cancer cells nevertheless resist an increase in their overall ploidy levels upon Twist1 overexpression (Fig. 2E, G–H). Here, PDXP is linked to colorectal cancer.