Further confirmation of the results using Western blotting showed that the anti-apoptotic protein pAkt was significantly upregulated and the apoptotic proteins such as Bax, cytochrome c and cleaved caspase 3 were significantly increased in the HF group mice however, IF administration and exercise treatment effectively reversed the HFD induced effects in both heart and liver tissues of SAMP8 mice (Figure 6). This evidence concerns the gene CASP3 and hydrops fetalis.