As a result of treatment with Lis, an increase in skeletal muscle fibrosis, as well as serum Ang II and TGF-β-Smad2/3 signaling in the skeletal muscle, was also prevented in the skeletal muscle of MI mice without affecting cardiac function, spontaneous physical activity, and blood pressure (Figs. 3, 4, and Table 1). Here, SMAD2 is linked to myocardial infarction.