Determination of HER2 gene status in breast cancer specimens has been performed at the HER2 gene copies level using Polymerase chain reaction, Southern blotting, Fluorescence in situ hybridization (FISH), Chromogenic in situ hybridization (CISH) or Dual-colour chromogenic in situ hybridization (Dual-ISH or DISH) (Furrer et al., 2015). This evidence concerns the gene ERBB2 and breast cancer.