Next, we provide evidence that combined anti-PD-1/G007-LK treatment of B16-F10 tumors is effectuated by G007-LK-induced loss of β-catenin in the tumor cells and induction of an interferon-γ (IFNγ)- and CD8+ T cell-dependent anti-tumor-immune response. This evidence concerns the gene PDCD1 and neoplasm.