Using the immunohistochemistry results from the Human Protein Atlas (HPA)47, ZDHHC-4/12/23 only expressed in nucleus and ZDHHC-22 did not express in both colorectal cancer (CRC) and cervical cancer (CESC), which did not match the subcellular distribution of GSDME (Fig. 7c, d). The gene discussed is ZDHHC22; the disease is cervical squamous cell carcinoma.