Using the immunohistochemistry results from the Human Protein Atlas (HPA)47, ZDHHC-4/12/23 only expressed in nucleus and ZDHHC-22 did not express in both colorectal cancer (CRC) and cervical cancer (CESC), which did not match the subcellular distribution of GSDME (Fig. 7c, d). Here, ZDHHC22 is linked to colorectal carcinoma.