In order to better define the role of FOXO1 and FOXO3 proteins in breast cancer, we also used the RPPA approach to study other proteins involved in the PI3K/AKT/mTOR oncogenic pathway, which regulates the activities of FOXO proteins, as well as various cellular processes involved in cancer and regulated by FOXO proteins: DDR, apoptosis, cell cycle, and cell proliferation (Table 4). The gene discussed is AKT1; the disease is breast cancer.